Journal: Mediators of Inflammation

Article Title: Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period

doi: 10.1155/2019/9515628

Figure Lengend Snippet: Decreases in CD25 expression/IFN- γ production on/in T-cell/PBMC of healthy donors (HD) and HAE patients after coculture with autologous neutrophils. (a, b) Coincubation of T-cells with autologous neutrophils in response to CD3/CD28 stimulation results in reduced percentages of CD25 + T-cells of total CD3 + CD4 + or CD3 + CD8 + T-cells in HAE patients. (c, d) Coincubation of T-cells with autologous neutrophils in response to the stimulation with CD3/CD28 results in reduced percentages of IFN- γ + T-cells of total CD3 + CD4 + or CD3 + CD8 + T-cells in HAE patients. (e, f) PBMC coincubation with autologous neutrophils in response to stimulating with CD3/CD28 results in reduced CD25 + T-cell percentages of total CD3 + CD4 + cells in HAE patients and CD3 + CD8 + T-cell in HD. (g, h) Coincubation of PBMC with autologous neutrophils in response to stimulation with CD3/CD28 results in reduced percentages of IFN- γ on CD3 + CD4 + or CD3 + CD8 + T-cells in HAE patients and HD too (horizontal bars represent medians; whiskers: 10-90 percentile; stars represent the statistical significance: ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, and ∗∗∗ p ≤ 0.001.

Article Snippet: T-cells were isolated from the peripheral blood using a RoboSep Human CD3 Positive Selection Whole Blood kit (STEMCELL Technologies).

Techniques: Expressing

Journal: Mediators of Inflammation

Article Title: Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period

doi: 10.1155/2019/9515628

Figure Lengend Snippet: Co-incubation of T-cells with autologous neutrophils in response to stimulation with CD3/CD28 resulted in reduced IFN- γ accumulation in culture supernatants in response to stimulation with CD3/CD28. Horizontal bars represent medians, analyzed using Wilcoxon or Mann-Whitney U test as appropriate; horizontal bars represent medians: ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, and ∗∗∗ p ≤ 0.001.

Article Snippet: T-cells were isolated from the peripheral blood using a RoboSep Human CD3 Positive Selection Whole Blood kit (STEMCELL Technologies).

Techniques: Incubation, MANN-WHITNEY

Journal: Blood

Article Title: Loss of heterozygosity 4q24 and TET2 mutations associated with myelodysplastic/myeloproliferative neoplasms

doi: 10.1182/blood-2009-02-205690

Figure Lengend Snippet: LOH 4q24 mapping and TET2 sequencing. (A) Topographical map illustrating overlapping regions of UPD4q24 (purple bars) and del4q24 (orange bars) in patients with myeloid malignancies. A minimally affected region on 4q24 (0.35 Mb), containing TET2 and PPA2, was identified in 18 of 398 patients studied (* indicates that some patients' DNA was not available for mutational screening; n = 3). (B) Representative examples of SNP-A karyograms (CNAG version 3.0) of chromosome 4 of both whole bone marrow cells and paired CD3+ lymphocytes in 2 patients, demonstrating the somatic nature of acquired LOH4q24 and TET2 mutations, which are shown in corresponding ferrograms in the right portion of the figure. SNP-A karyograms: rows a and b correspond to the copy number as measured by the intensity of individual hybridization signals; row c represents frequency of heterozygous calls; and row d indicates the allelic imbalance with parental allele copy numbers.

Article Snippet: Baseline patient characteristics SNP-A analysis DNA was extracted from patient marrow specimens and, when sample material allowed, CD3 + cell fractions were isolated using RoboSep (StemCell Technologies, Vancouver, BC).

Techniques: Sequencing, Hybridization

Journal: Leukemia & lymphoma

Article Title: Cord Blood Chimerism And Relapse After Haplo-Cord Transplantation

doi: 10.1080/10428194.2016.1190970

Figure Lengend Snippet: Day 56 Chimerism and Transplant outcomes and 95% CI-Significant differences highlighted

Article Snippet: 4 Chimerism Assessment CD3 positive and CD33 positive cells were isolated from post-transplant peripheral blood (PB) samples using magnetically-labeled anti-human CD3+ or CD33+ antibodies (isotypes: mouse IgG1 and IgM kappa, respectively) and the fully automated RoboSep® cell separator (STEMCELL Technologies Inc., Vancouver, Canada).

Techniques:

Journal: Leukemia & lymphoma

Article Title: Cord Blood Chimerism And Relapse After Haplo-Cord Transplantation

doi: 10.1080/10428194.2016.1190970

Figure Lengend Snippet: A. Cumulative Incidence of Relapse based on D56 UCB CD3 Chimerism AML-MDS

Article Snippet: 4 Chimerism Assessment CD3 positive and CD33 positive cells were isolated from post-transplant peripheral blood (PB) samples using magnetically-labeled anti-human CD3+ or CD33+ antibodies (isotypes: mouse IgG1 and IgM kappa, respectively) and the fully automated RoboSep® cell separator (STEMCELL Technologies Inc., Vancouver, Canada).

Techniques: